为了探究碳点（CDs）在生物传感方面的应用，以季也蒙毕赤酵母菌（Meyerozyma guilliermondii PG-1）为原料，通过一步水热合成法成功制备了水溶性良好、光学性质稳定的蓝色荧光碳点，并通过透射电子显微镜、傅里叶红外光谱、X射线电子衍射和荧光光谱等方法对CDs进行表征。获得的CDs近似圆形，尺寸在11 nm左右，表面含有大量亲水性官能团，432 nm处有最强的发射光谱。进一步将CDs用于水中微量盐酸四环素（TC）的检测。结果显示，基于静态猝灭和内滤效应，TC能够快速猝灭碳点荧光，荧光猝灭效率在0.02～0.18 g·L-1浓度范围内有良好的线性关系，表明碳点作为荧光探针检测TC的潜在应用前景。
In order to explore the application of carbon dots (CDs) in biosensors, this paper successfully prepared blue fluorescent carbon dots with excellent water solubility and stable optical properties by one-step hydrothermal synthesis using Meyerozyma guilliermondii PG-1 as raw material, and characterized CDs by transmission electron microscopy, Fourier infrared spectroscopy, X-ray electron diffraction and fluorescence spectroscopy. The obtained CDs were approximately round, with a size of about 11 nm, a large number of hydrophilic functional groups on the surface, and the strongest emission spectrum at 432 nm. CDs was further applied to the determination of trace tetracycline hydrochloride (TC) in water. The results showed that TC can rapidly quench the fluorescence of carbon dots based on static quenching and internal filtering effect, and the fluorescence quenching efficiency has a good linear relationship in the concentration range of 0.02-0.18 g·L-1, indicating its potential application as a fluorescent probe for TC detection.