有机磷杀虫剂已被广泛用于控制农业害虫，其在农产品与环境样本中的残留引起人们的关注。课题组前期筛选了一株有机磷杀虫剂高效降解菌株，南通嗜铜菌X1T（Cupriavidus nantongensis X1T）。研究了菌株X1T对手性有机磷杀虫剂丙溴磷对映体在不同底物浓度、菌量、pH、金属离子条件下的降解特性。结果表明，菌株X1T可以优先选择性降解高毒低效对映体S-丙溴磷。随着底物浓度的升高，菌株X1T对丙溴磷对映体的降解速率逐渐减慢；菌株X1T降解丙溴磷的最适pH为7.5；金属离子价态是影响丙溴磷降解的重要影响因素，其中Cr3+与 Fe3+等3价金属阳离子显著抑制菌株对丙溴磷降解，Ca2+、Mn2+、Mg2+、Co2+、Ni2+等2价金属阳离子显著促进菌株对S-丙溴磷降解。同时，菌株X1T中降解酶粗酶与异源表达的纯酶对丙溴磷手性对映体的降解速率无显著性差异。结果为丙溴磷残留污染的生物修复技术提供理论依据。
Organophosphorus insecticides (OPs) have been widely used to control agricultural pests, which has raised concerns about OPs residues in crops and the environment. The research group had previously isolated a highly efficient OPs degradation strain , Cupriavidus nantongensis X1T. In this study, the degradation characteristics of chiral organophosphorus pesticide profenofos by strain X1T under different substrate concentrations, bacterial counts, pH and metal ions were measured. The results showed that strain X1T could preferentially degrade S-profenofos with high toxicity and low insecticidal activity. With the increase of substrate concentration, the degradation rate of profenofos by strain X1T gradually slowed down the optimal pH of profenofos degradation by strain X1T was 7.5; the valence state of metal ions was an important factor to affect the degradation of profenofos, of which Cr3+ and Fe3+ could inhibit the degradation of profenofos, while Ca2+, Mn2+, Mg2+, Co2+, Ni2+ could promote the degradation of S-profenofos. Meanwhile, there was no significant difference between the degradation rate of S-profenofos and R-profenofos by the crude enzyme in strain X1T and purified enzyme opdB. The results provide a theoretical basis for bioremediation of profenofos residuals.