To investigate the influence of host plant nutrients in bark to host selection of Anoplophora chinensis (CLB), and host selection principle of CLB, 8 tree species (varieties) were selected which are often damaged by CLB to determine the feeding and habitat selection, oviposition preference of CLB, and the contents of inclusions in bark were also determined. The results were as follows: CLB tended to feed on Acer negundo, A. saccharum and A. palmatum, preferred inhabit and oviposit in A. negundo, A. saccharum and M. azedarach, seldom fed, inhabited and oviposited on Q. nuttallii and S. babylonica. Correlative analysis showed that the feeding area, inhabiting and oviposition number of CLB had significantly positive correlation with total amino acid content, amino acid content in bark, and significant negative correlation with carbon-nitrogen ratio, but uncorrelation to soluble saccharide, soluble protein, tannin and total phenolics content in bark. Feeding amount of CLB had no significant correlation to the bark nutrients we had determined. Carbon-nitrogen ratio and amino acid content were important factors affecting feeding, inhabiting and oviposition selection of CLB. Appropriate carbon-nitrogen ratio and high amino acid content is one of the standards of host selection of CLB.