In order to detect the expression of microRNA-222-3p in different tissues of feeding geese to predict and validate the target genes of microRNA-222-3p and to explore the function of microRNA-222-3p in the formation of goose fatty liver. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-222-3p in the liver, pectoral muscle and abdominal fat of goose, bioinformatics was used to predict the target gene of microRNA-222-3p, quantitative fluorescence PCR was used to detect the predicted target gene expression in liver, and double luciferase gene reporting system was used to verify the target gene. The results showed that the expression of microRNA-222-3p was significantly increased in both liver and abdominal fat of feeding geese compared with the control group, the expression of bioinformatics predictions showed that MARF1 and B4GALNT3 had potential binding sites of microRNA-222-3p in the 3'UTR region, and both genes were significantly down-regulated in the fatty liver of geese. However, the dual-luciferase gene reporting system showed that only MARF1 was the target gene of microRNA-222-3p in geese. The results indicated that microRNA-222-3p expression was significantly up-regulated in goose fatty liver, and may play a regulatory role in the formation of goose fatty liver through its target gene MARF1.