Oocyte maturation is regulated by trimethylation of Lysine 4 on histone 3 and trimethylation of Lysine 27 on histone3. Oocyte maturation is also regulated by H3k4me3 and H3K27me3’s methylation and demethylase. So it is considered that there is also a certain impact on the development of chicken’s follicles. For the object of exploring the expression pattern of H3K4me3 and H3K27me3 in different developmental stages of chicken’s follicular granular layer, the female line of Suqin 3 was used as experimental animal. The result showed that, in the follicular granular layer of Suqin 3, the expression pattern of H3k4me3 at different stages of follicle development showed a wavy trend of decreasing → increasing → decreasing → increasing, and the wave changes were relatively gentle. The expression level of the three high expression points of F5, F2 and F1 was significantly different from that of the four low expression points of SWF (small white follicle), LWF (large white follicle), SYF (small yellow follicle) and F3 (P<0.05). The expression pattern of histone H3K27me3 at different developmental stages of follicular granular layer also shows a wavy expression trend, bur with obvious fluctuations. The expression of SWF, SYF and F3 was significantly higher than the expression of d F5, F4, F1 and F2 (P <0.05). Correlation analysis showed that the expression of H3k4me3 and H3K27me3 in follicular granulosa cells at different developmental stages showed a strong negative linear correlation (R=-0.808, P=0.000). Result prompt: The expression of H3k4me3 and H3K27me3 in the follicular granulosa layer at different stages is tissue-specific and negatively correlated with dynamic modification,