In order to further optimize the technical system of tissue culture and seedling raising of Actinidia chinensis cv. Hongyang, the stem tips and leaves of sterile tissue culture seedlings of A. chinensis cv. Hongyang were used as the materials, and ZT 1.5 mg·L^-1 was used as the plant growth regulator to screen a suitable basic medium, and then the effects of different combinations of plant growth regulators on indirect and direct organogenesis and plant regeneration were studied. The results showed that: the optimum medium for the growth of A. chinensis cv. Hongyang was OM. In the medium of OM + 2.0 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 0.01 mg·L^-1 2, 4-D, the callus induction rate was 100%, and the adventitious bud generation coefficient was 3.68; the stem tips were cultured in OM + 1.5 mg·L^-1 6-BA + 0.5 mg·L^-1 NAA + 1.0 mg·L^-1 KT for 60 days, the basal stem cluster buds were produced by direct organogenesis, and the proliferation coefficient could reach 7.65. The optimal rooting medium for test-tube seedlings was 1/2 OM+NAA 0.5 mg·L^-1; after 60 days, the average number of adventitious roots was 4.87, and the survival rate of domesticated seedlings was more than 90%. The basic medium suitable for the growth of A. chinensis cv. Hongyang was selected, and the problems of leaf yellowing, dwarf and deformity of test-tube plantlets were solved, and the proliferation coefficient was greatly increased. The artificial rapid propagation system of A. chinensis cv. Hongyang was optimized to provide a reference for the research of other kiwifruit varieties.