In order to study the effect of lead pollution on the fungal diversity in yellow cinnamon soil, the simulated culture experiment (28 days) was used to detect the community composition, abundance and culturable population of fungi in yellow cinnamon soil under different concentrations of lead by using Acer sequencing technology and Dilution plate method. The results showed that the inhibitory effect of lead stress on the fungal community was enhanced with the increase of lead concentration and weakened with the prolongation of culture time. High-concentration lead treatment (2 500 mg·kg^-1) significantly inhibited the number of culturable fungi throughout the culture period. In the early stage of culture (1 - 7 d), the OTU and diversity indexes of soil fungi in the control treatment were the highest, but in the middle and late culture period (14 - 28 d)，the OTU and diversity indexes of fungi in the middle-concentration lead (500 mg·kg^-1) treatment were the highest. The fungal diversities of the high-concentration lead treatments (2 500 mg·kg^-1) were the lowest during the whole culture period. Fourteen fungal phyla were detected at the phylum level. Ascomycota was the first dominant flora, followed by Mortierellomycota (except for the high-concentration lead treatment when cultured for 1 d) and Basidiomycota. When lead was added for 1 day, high-concentration lead treatment significantly decreased the abundance of Ascomycota, while which significantly increased the abundance of Basidiomycota. During the culture period of 7 - 28 days, the abundance of Ascomycota increased with the increase of lead concentration, while Basidiomycota increased first and then decreased. The addition of lead had little effect on the abundance of Mortierellomycota. The abundance of unknown fungal