The leaves, barks and flowers of Hibiscus tiliaceus are the medicinal parts of the traditional Chinese medicine H. tiliaceus, which have the medicinal values of antibacterial, anti-inflammatory and anti-tumor. There is information transmission between medicinal plant endophytic fungi and host plants at the genetic level, which can produce the same or similar metabolites as host plants, so H. tiliaceus endophytic fungi may become one of the sources of new natural products. In this study, 64 strains of endophytic fungi were isolated and identified from different parts of semi-mangrove plant H. tiliaceus, fermented in solid state, and then oxford cup method was used to detect the bacteriostatic activities of the metabolites to five kinds of common pathogenic bacteria. According to different construction of phylogenetic tree of the separate parts, three strains had been screened to be inhibitor activity of bacteria, which can be used to study the chemical composition of metabolites in the future.