A pot experiment was performed to study the Ciprofloxacin (CIP) content in the tissue of Chinese cabbage (Brassica parachinensis L.) and the characteristics of physiological and biochemical treated with four different nitrogen forms (amide nitrogen, nitrate nitrogen, ammonium nitrogen and cyanamine nitrogen). The influence mechanism of different forms of nitrogen fertilizer on absorption and transport capacity of CIP in Chinese cabbage was discussed based on the perspective of physiology and biochemistry. The results showed that all nitrogen fertilizers significantly reduced the CIP content in Chinese cabbage tissue, and significant differences were in the CIP enrichment among four nitrogen forms. The CIP content in the shoot and root of Chinese cabbage was in the order of amide nitrogen < ammonium nitrogen < nitrate nitrogen < cyanamide nitrogen, which decreased by 20.7% - 62.0% compared with the control group. The root enrichment coefficient (BCF) and the stem leaf transport coefficient (TF) of Chinese cabbage were reduced by nitrogen fertilizer, and among which amide nitrogen and ammonium nitrogen had the most significant inhibitory effects on BCF and TF. At the same time, the application of nitrogen fertilizer significantly increased the biomass, nitrate reductase activity and chlorophyll (a and b) content of Chinese cabbage leaves. Combined with the analysis of fluorescence dynamic curve and chlorophyll fluorescence parameters of leaf