为研究独角仙雄虫水提取物对小鼠的镇痛作用，以水提法处理独角仙雄虫粉末，将ICR小鼠随机分成空白对照组、阳性药阿司匹林组和独角仙雄虫水提取物低、中、高浓度组，采用醋酸致小鼠扭体法、热板法和小鼠甩尾法研究其对醋酸致小鼠扭体次数和小鼠痛阈值的影响。结果表明：低、中、高浓度组小鼠扭体次数分别为（11.17 ± 5.77）次、（15.00 ± 6.00）次和（11.83 ± 4.57）次，低浓度组镇痛百分率达到51.43%，与模型对照组相比，独角仙雄虫水提取物低、中、高浓度组都能明显降低醋酸对小鼠扭体次数的影响，提升了小鼠痛阈值；延长小鼠在热板和甩尾实验中的耐热时间。研究结果证明独角仙雄虫水提取物对小鼠确实具有一定的镇痛作用且对小鼠无毒副作用，为之后独角仙入药及新型镇痛药物的开发提供一定的理论依据。
In order to investigate the analgesic effect of aqueous extract of male rhinoceros beetle on mice, male rhinoceros beetle powder was treated with aqueous extract, and ICR mice were randomly divided into blank control group, positive drug aspirin group, aqueous extract of male rhinoceros beetle with low, medium and high concentrations. The effects of acetic acid on the number of acetic acid torsions and the pain quotient of mice were investigated by the acetic acid-induced writhing, tail-flicking and hot plate methods. The results showed that the writhing times of mice in low, medium and high concentration groups were（11.17 ± 5.77）,（15.00 ± 6.00）,（11.83 ± 4.57）respectively, and the percentage of analgesic in the low concentration group reached 51.43%. Comparing with the model control group, the low, medium and high concentration groups of male rhinoceros beetle aqueous extract could significantly reduce the effect of acetic acid on the number of mouse writhing times, and enhance the mice's pain threshold; prolonged the heat resistance time of mice in hot plate and tail-flick experiments.This experiment results proved that the aqueous extract of male rhinoceros beetle did have certain analgesic effects on mice without toxic side effects, which provided some theoretical basis for the development of rhinoceros beetle as medicine and new analgesic drugs in the future.