In order to simulate and study the affecting factors for the biomass of wheat in its growth process, 15 scenarios were designed using the WOFOST crop model, and the sensitivity parameters of the model were screened to simulate and evaluate the influence of soil moisture nutrients on wheat biomass using scenario analysis and systematic analysis. The results showed that 6 parameters in WOFOST model were extremely sensitive with sensitive indices above 0.1, including the effective light energy utilization rate of single leaf under 20℃(EFFTB3), relative leaf area at 0.0 and 0.5 of set growth stages index(SLATB1 and SLATB2), accumulated temperature from seedling to flowering stage（TSUM1）, maximum CO₂ assimilation rate at 30 ℃(TMPF4), and growth cycle of leaf area at 35 ℃(SPAN）.It was verified that these 6 parameters in WOFOST wheat model .LAI, TAGP and TWSO were simulated for biomass indicators under the condition of nutrient deficiency, normal nutrient and nutrient fertility, and it was concluded that soil nutrients had little impact on wheat biomass, while soil moisture had a greater impact on wheat biomass. Based on simulation results, the saturation value of AWC was 190 (kg·m^-1), and the wheat yield increased with the increase of soil water, but when water reaches saturation value, the increase of water has little effect on the wheat yield. This study can provide a scientific theoretical basis for parameter optimization and regional application of WOFOST wheat model, as well as quantitative simulation of the influence of soil water and nutrient on wheat yield.