Based on bioinformatics, the phylogenetic information of 16 species of silkworm moths was analyzed by using the base sequence of COX1 as a molecular marker. The results showed that the nucleotide sequence of COX1 gene has obvious AT bias, and 26 codons in the codons of coding amino acids with obvious usage bias. The corrected genetic distance among populations was within the range of 0.003-0.177, indicating that silkworm moths have close genetic relationships. Using MEGA6 software, the phylogenetic tree was constructed by neighbor-Joining (NJ) and maximum likelihood (ML), respectively. The branches of the development tree were obvious, and most of the node support rates were very high, further indicated that the relationship between silkworm moth insects is closer. This study supplements the knowledge of the traditional classification of silkworm moths and provides a basis for the classification of silkworm moths.