In recent years, ecological environmental protection has become an important topic of sustainable development. This paper used the land use classification of remote sensing and geographic information system to extract the land use distribution characteristics of Yangshuo county in Guilin city in 2000, 2008 and 2016, calculated the land use landscape pattern index, land use conversion matrix, etc., combining Yangshuo County ecosystem service value, and analyzed the status and changes of land use and its impact on the ecological environment in Yangshuo county. The results showed that the construction land had the largest change and the change of forest land was small in Yangshuo county from 2000 to 2016; the conversion of cultivated land and forest land to construction land was more in the conversion of different land use types, which was one of the main sources of urban land use; the landscape pattern showed a trend from aggregation to fragmentation and diversification from 2000 to 2016; the total value of ecosystem services increased by 53.88 million yuan between 2000 and 2016, indicating that the ecological environment is moving towards a healthy and friendly direction.