In order to study the interaction of combinations of three plant growth regulators (GGR, IAA and PP₃₃₃) on the growth and resistance physiological of Tabebuia chrysantha seedlings, and obtain the best concentration combination for improving the resistance of Tabebuia chrysantha seedlings, we chosen Tabebuia chrysantha seedlings as experimental materials, used L₁₆(4⁵) orthogonal experiment design, measured and compared the growth and physiological characteristics of Tabebuia chrysantha seedlings. The results showed that root growth index and growth amount of seedling height with No. 11 treatment (GGR 400 mg·L^-1, IAA 400 mg·L^-1, PP₃₃₃ 400 mg·L^-1) was greater than those with other combination treatments, and which had the largest growth ground diameter with No.8 treatment, followed by No. 11. Among the three plant growth regulators, GGR played a leading role in the root growth index, growth amount of seedling height and diameter of Tabebuia chrysantha seedlings, but IAA and PP₃₃₃ had no significant effect. The soluble protein and superoxide dismutase (SOD) activity in the seedlings with No. 11 treatment were greater than those with other treatment groups, while the content of malondialdehyde (MDA) in the seedlings with No. 11 treatment was the lowest, and the activities of peroxidase (POD) in the seedlings with No. 4, No. 11 and No. 12 treatments were significantly higher than those with other treatments. The results showed that: GGR plays a leading role in the soluble protein, SOD activity and POD activity of the Tabebuia chrysantha seedlings,