This study compared the sensitivity and accuracy of detection on feline herpesvirus-1 by using three methods of traditional PCR, Real-time?PCR and colloidal gold detection card. Two methods of traditional ?PCR and Real-time PCR based on Taq Man probe technology were established to detect feline herpesvirus-1 in?our lab. The minimum of copies detected by fluorogenic quantitative PCR and traditional PCR were 20 and 207, respectively. The two well?established?methods?in?our?lab?and?the?rapid?detection?card?of Feline herpesvirus-1 were used to detect 21 secretion?suspected?Feline?herpesvirus?infection?clinically. It?showed that the detection rates of Real- time PC, traditional?PCR?and?quick?test?card?were?71%,57%?and?29%, respectively. The result indicated that compared with the rapid detection card of cat herpesvirus type 1, the detection method of traditional PCR and Real- time PCR is?more?sensitive?and?accurate, especially?Real-time?PCR which can provide more accurate ?results for clinical diagnosis.