To explore the difference in HCN and tar yields in the flue-cured tobacco leaf from different growing areas, leaves from different stem locations of 60 tobacco cultivars collected from the main tobacco growing areas were analyzed using the ANOVA method. The results showed that the yield of tar and HCN was influenced by the leaf location on the stem with a significant difference at the 0.05 level. The upper leaves contained the highest yield of tar and HCN, followed by the leaves in the middle and low portion of a stem. The difference of HCN in the flue-cured tobacco leaf from different growing areas was not significant; however, the dufference in the tar yield was significant at the 0.05 level. The interaction among the growing area, leaf location, and cultivars was not significant for thw yield of tar and HCN. Therefore, the flue-cured tobacco leaf location on the stem should be considered firstly and the growing area and cultivar should be also considered for the raw material selection, mixture and storage.