Using the protocorm-like body (PLB) of Cymbidium hybridum, the effects of culture time, basic medium,?sugar concentration,?cultivation form,?and?cutting?way on proliferation,?differentiation and?conservation in vitro?were studied. The results showed that the PLB?proliferated?and differentiated in two months culture with the differentiation?being more effective after two months culture. Half-strength MS medium?was beneficial to?PLB?proliferation and differentiation,?while 3 MS?medium was favorable to?PLB preservation. Sucrose at 20 g·L^-1 was good for?PLB?preservation,?while 50 g·L^-1 sucrose?was suitable for?PLB proliferation. Sucrose at 30 g·L^-1 was effective for PLB differentiation. Solid culture had an obvious effect for PLB differentiation,?while liquid culture?was good for PLB proliferation.?PLB conservation in vitro could use static liquid culture. The sheet-cutting method?was suitable for?PLB?proliferation and differentiation?and the whole-peeling method was good for?PLB?preservation. The best PLB proliferation condition was 1/2 MS+50 g·L^-1 sucrose +2.0 mg·L^-1 6-BA +0.1 mg·L^-1 NAA in liquid culture with the sheet-cutting method. The best PLB differentiation condition was 1/2 MS+ 30 g·L^-1 sugar in solid culture with the sheet-cutting method. The best PLB conservation condition was 3 MS+ 20 g·L^-1 sucrose in liquid culture with the whole?peeling method. It indicated that this method is effective for rapid propagation and conservation of Cymbidium hybridum in vitro.