The expression of F gene of Newcastle disease virus (NDV) was analyzed using the baculovirus expression system. HN segments were amplified using RT-PCR and cloned into pFast BacHT A plasmid. The recombinant plasmid was transformed into DH10Bac competent cells to get the positive recombinant bacmid. The expression of the target gene was detected after 72 h post transfection of Sf9 cell with recombinant bacmid. Serum antibody titer of immunized SPF chickens and the protective properties were determined. The indirect immunofluorescence assay (IFA) and western-blot test showed that the HN gene expressed in Sf9 cells infected with the recombinant baculovirus. The ELISA results showed that the expressed protein could induce high titer specific antibody against NDV. The protection rate of JSXZ NDV strong virulent strains was 75%, and the protective rate in the La Sota vaccine group was significantly higher than that in the negative control group. These results would lay a foundation for the further study on Newcastle disease virus subunit vaccine.