In order to optimize the extraction process of phenylethyl alcohol glycosides from Cistanche tubulosa, the effects of ethanol extraction, ultrasonic extraction, and microwave extraction on the yield ratio of phenylethyl alcohol glycosides were studied using response surface analysis (RSA). The antioxidant capacity of phenylethyl alcohol glycosides was determined by in vitro antioxidant analysis using DPPH method. As results, the microwave extraction method showed the highest yield of benzyl ethanol in the shortest time followed by the ultrasonic extraction method. The ethanol extraction method showed the lowest efficiency with the longest processing time. With the increase of the phenylethyl alcohol glycosides concentration, the DPPH radical scavenging capacity was also increasing and its?maximum value occurred at the concentration of 60-70 μg·mg-1. This study provides valuable information to improve the extraction process of phenylethyl alcohol glycosides from Cistanche tubulosa, which will benefit the research on the pharmacological and biological activity research of this compound.