In order to study the effect of Chinese herbal anticoccidial additive on Eimerial tenlla in chickens, 150 14-day old chickens were randomly divided into groups of negative control, positive control, 1%, 2%, and 5% of Chinese herbal additive treatment. The chickens in the two control groups were fed with basic diet and those in the treatment groups were fed with basic diet supplemented with 1%, 2%, or 5% of Chinese herbal additive. Except the chickens in the negative control group, each chicken was vaccinated against 5.0×10⁴ sporulated Eimeria coccidian oocysts. The anticoccidial index was calculated using the survival rate，relative body weight gain，oocyst value, and lesion value. The inhibitory effect on oocysts sporulation rate was estimated using in vitro culture of coccidian oocysts isolated from chicken intestine. The results showed that the Anticoccidial indexes (ACI) of 2% Chinese herbal additive group was 169 and had the maximum inhibitory effect on in vitro oocyst sporulation.