为研究中草药添加剂对鸡柔嫩艾美尔球虫的抗球虫效果， 将14日龄的鸡150只，随机分为阴性对照组，阳性对照组，1%、2%和5%中草药添加剂组。对照组饲喂基础日粮，给药组在基础日粮的基础上分别添加1%、2%和5%中草药添加剂。除阴性对照组外，每只鸡接种孢子化艾美尔球虫卵囊5×104个。根据成活率、相对增重率、卵囊值、病变计算抗球虫指数，并对肠道分离的球虫卵囊进行了体外的孢子化培养，评价对球虫卵囊孢子化的抑制作用。结果显示， 2%中草药添加剂抗球虫指数为169，对体外卵囊孢子化抑制作用最高。
In order to study the effect of Chinese herbal anticoccidial additive on Eimerial tenlla in chickens, 150 14-day old chickens were randomly divided into groups of negative control, positive control, 1%, 2%, and 5% of Chinese herbal additive treatment. The chickens in the two control groups were fed with basic diet and those in the treatment groups were fed with basic diet supplemented with 1%, 2%, or 5% of Chinese herbal additive. Except the chickens in the negative control group, each chicken was vaccinated against 5.0×104 sporulated Eimeria coccidian oocysts. The anticoccidial index was calculated using the survival rate，relative body weight gain，oocyst value, and lesion value. The inhibitory effect on oocysts sporulation rate was estimated using in vitro culture of coccidian oocysts isolated from chicken intestine. The results showed that the Anticoccidial indexes (ACI) of 2% Chinese herbal additive group was 169 and had the maximum inhibitory effect on in vitro oocyst sporulation.