Gene between viruses and plants is the molecular basis of plant susceptibility and resistance to virus diseases. In this study, three RNA viruses (potato virus Y-PVY, tobacco mosaic virus-TMV, and cucumber mosaic virus-CMV) were used to study virus-induced transcription factor genes in Nicotiana tabacum. These genes may play an important role in plant defensive responses to virus infection. Based on public database and literature, ten tobacco genes homologous to the virus-induced genes previously identified in N. benthamiana and Oryza sativa were found. Quantitative analyses of the gene transcripts responding to virus infection, abiotic stresses, and stress related compounds (1 mmol·L^-1 of methyl jasmonic acid or salicylic acid solution) revealed that each virus had its specific expression profile of induced plant genes. Tobacco 06G gene, a member of WRKY subfamily II, was quickly and effectively induced by all three viruses. Expression of the 06G gene was higher in young leaves than in old ones. Our data suggest that this novel WRKY gene may function as a regulator in plant-virus interactions and such interactions can be utilized to enhance virus resistance in plants.