In this paper, semi-lignified stems were used to initiate an in vitro culture of Lonicear macckii ‘Aoxue’. Effects of surface sterilization methods and plant growth regulators on induction of adventitious buds, shoot multiplication, and rooting were determined. The results indicated: (1) Low contamination and browning of stem explants were observed when they were surface-sterilized with 75% ethanol for 30 s followed by washing with sterile water for 4-6 times, and then soaking in 0.1% mercuric chloride (HgCl₂) for 7 min; (2)The best medium for shoot proliferation was MS + 6-BA 1.0 mg·L^-1+ NAA 0.15 mg·L^-1+ GA₃ 0.5 mg·L^-1, with a proliferation rate of 2.93; (3)The rooting rate of plantlets was up to 93.9% in ? strength of MS medium supplemented with 1.5 mg·L^-1 IBA. All media used contained 30 g·L^-1 sucrose and 7 g·L^-1 agar. The medium pH was adjusted to 6.0 before autoclaving.