Four primers were synthesized to amplify nucleic acid sequences (a tandem repeat consisting of three IBDV multiple epitopes DNA sequence) by SOE PCR (splicing overlap extension PCR method). The nucleic acid sequence digested by EcoR I and Sal I was inserted into the expression vector pET32a. SDS-PAGE results showed that the recombinant multiple epitopes peptide (tandem peptide?of three IBDV epitopes) comprised about 20 % of the E.coli total protein. Agarose diffusion assay suggested that recombinant multiple epitopes peptide was demonstrated to have good antigenicity.