The callus culture of tea plant provides a good platform for the regulation and metabolism of secondary metabolites in the tea plant. This research carried out the work in screening for tea cell lines containing different catechin contents based on the callus culture of the tea plant. The results showed that among H, S and B induction mediums, only B medium was fitted for inducing and screening callus which were quickly grown, loosely organized, even textured and produced a good amount of catechins. Callus induced from tender stems and leaves grew quickly and loosely, but its regeneration ability to plants was weak. Cell lines “yunjing63Y”, “yunjing63X” with different catechin contents were obtained by the visual method, dichotomy, and HPLC analysis. The results showed that catechin components in the two cell lines were similar to those in the fresh tea leaves, but the catechin contents in cell lines “yunjing63Y”and “yunjing63X” differed obviously. The catechin contents (DW) of the former was 7.77 mg·g^-1, and the latter was 0.13 mg·g^-1.