The effects of different maturity on content of aroma in Guizhou organic tobacco were studied. The results showed that the content of aroma in the middle leaf of organic tobacco changed obviously with the variation of maturity, it descended sharply with the increase of maturity and then rose slowly at the stage of full maturity, which was biggest at basic maturity. The aroma content in the upper leaf of organic tobacco increased slowly from basic maturity to maturity and then decreased slowly with the increase of maturity, and it was biggest at maturity. The amplitude of variation of neophytadine, degraded carotenoid products, maillard reaction products and cembrenoid products were similar to the neutral aroma in the middle leaf of organic tobacco. The amplitude of variation of neophytadine, maillard reaction products and cembrenoid products were similar to the neutral aroma in the upper leaf of organic tobacco. The content of aroma was biggest in middle leaf of organic tobacco at basic maturity or in upper leaf of organic tobacco at maturity.