以映山红(R. simsii）、马银花(R. ovatum)、满山红(R. mariesii)和云锦杜鹃(R. fortunei)4种杜鹃为实验材料，采用TCA-丙酮法、酚法和TCA-丙酮-酚法3种蛋白质提取方法提取杜鹃叶片蛋白质，并应用双向电泳技术（2-DE）比较分析不同方法的蛋白质提取率、溶解性、完整性等的差异，确立适用于2-DE分析的杜鹃叶片蛋白质提取方法。结果表明，TCA-丙酮法提取的蛋白溶解性差且粘稠，得到的蛋白质点少，不适合杜鹃叶片蛋白的提取；酚法提得的蛋白质溶解性好，蛋白质点多，但是杂质较多，且此法不适用于映山红叶片蛋白的提取；TCA-丙酮-酚法提取的杜鹃叶片蛋白质位点较多且清晰，重复性也好，且4种杜鹃叶片蛋白的提取效果都比较好。因此，TCA-丙酮-酚法被认为最适于杜鹃属植物叶片蛋白质的提取及分析。
In this study, three precipitation protocols of protein extraction for 2-DE proteomic analysis were used to extract the protein from leaf tissue of four Rhododendron species, and the extraction efficiency, solubleness, integrity of protein extractions were compared. The results indicated that the TCA-acetone protocol for protein extraction cannot be used to the Rhododendron.L because the extracted proteins were ropy and difficult to be redissolved. Though extracted proteins with phenol protocol were eutectic and the spots in the 2-DE maps were abundant, however the impurities were too much and the method is inapplicable to R. simsii. The TCA-acetone-phenol protocol displayed the best result with spot focusing, resolved spots, spot intensity, unique spots detected, and reproducibility，and is applicable to four Rhododendron species. So TCA-acetone-phenol protocol is considered the best protocol of the protein extraction for 2-DE proteomic analysis of Rhododendron leaves.