Polymerase chain reaction single-stranded conformational polymorphism (PCR-SSCP) analysis was used to identify the exon 1 of myostatin gene (MSTN) mutation in Wandong, Wannan, Wagyu and Simmental cattle populations. The results showed that an A→G mutation was occur at position 267 in exon 1 which caused no amino acid change, and 3 types of genotypes (AA and AG, GG) were found. A allelic gene was superiority in the cattle populations. It was moderate polymorphisms in the tested 4 populations. The chi-square test showed that Wandong, Wannan and Wagyu cattle populations in Hardy - Weinberg equilibrium (P>0.05) and Simmental in Hardy - Weinberg disequilibrium (P>0.05).