In this study, digesting and separating piglets liver cells obtained from modified shear digestion (1% Ⅳcollagenase )were cultured and dynamically observed with inverted microscopy, so as to establish a stable system for primary culture of piglets liver cells. RNAi and RT-PCR technique were employed to the detection of the cell mRNA expression level, and then the changes of THRSP mRNA expression at the cellular level were explored. The results showed that 6.2×10⁶ cells can be obtained from per gram of liver on the average, with seeding survival rate of 94.84% and the culture survival rate of about 50%, and transfected cell viability was more than 80%. The interfered efficiency of chemosynthetic siRNA was 75.3%.