Super high-yielding rice variety Fengliangyouxiang 1 was used to analyze the impact of different nitrogen application rates on group characteristics, nitrogen agronomic utilization and functional leaf senescence of two-line medium indica hybrid rice under the condition of conventional field test. The results showed as follows: when the nitrogen rate was in the range of 0-255kg·hm^-2, two-line hybrid rice production, population quality and nitrogen agronomic utilization increased along with the increase of nitrogen application rate; when the nitrogen application treatment was of 255 kg·hm^-2, it had the highest yield (11786.4 kg·hm^-2) and the maximum nitrogen agronomic utilization; when the nitrogen rate increased from 255 to 300 kg·hm^-2, the production, population quality and nitrogen agronomic utilization decreased. Rice aging process was affected by nitrogen nutrition. The optimum nitrogen application rate (255 kg·hm^-2) and a relatively high nitrogen agronomic utilization could ensure that the functional leaves of the two-line medium indica hybrid rice would not premature aging after the full heading, which is favorable for enhancing plant photosynthetic capacity and the accumulation of photosynthetic products at later stage, increasing the material accumulate contribution rate in later period, so as to lay a foundation for high yield.