In order to investigate the optimal dietary requirements of protein, lipid and carbohydrate for juvenile gibel carp (Carassius auratus gibelio) with initial average weight of (2.85±0.09) g, three-factor second order general revolting combination design was used, the high and low designed levels of protein, lipid, carbohydrate in the diets were of 33% and 26%, 10.5% and 5.5%, 40% and 30%, correspondingly. The experiment lasted for 60 days. The results showed as follows: when WGR(weight gain rate) and SGR (specific growth rate) were biggest but FCR (feed conversion ratio) was smallest, the corresponding dietary protein, lipid, carbohydrate levels followed by 35.05%, 8.23% and 27.65%, 35.62%, 8.63%and 28.16%, 37.15%, 4.56% and 31.48%, respectively. When the above three factor levels were of 33.22%, 5.81% and 25.58%, respectively , VSI (viserosomatic index) was smallest. No significant difference was observed in CF (condition factor)and HSI (hepatosomatic index )among all the treatments (P>0.05). There was a negative correlation between moisture content of whole-body and dietary lipid level (P<0.05), but a positive correlation between lipid content of whole-body and dietary lipid, carbohydrate levels (P<0.05). When the dietary lipid was above 8%, the lipid content of whole-body was increased obviously. No significant difference was observed in protein and ash content of whole-body (P>0.05). In this study, the optimal dietary requirements of protein, lipid and carbohydrate for juvenile gibel carp are of 35.05%-37.15%, 4.56%-8.00% and 27.65%-31.48% respectively by comprehensive analysis.