To explore the effects of different frozen-thawed methods on boar sperms, two ways of freeze and thaw the porcine sperms were used. Isolating the sperm acrosomal membrane protein, detecting total proteins by SDS-PAGE electrophoresis, and analyzing the total gray value were conducted. The result showed that the boar sperm viability of freezing and thawing with one-time dilution method was higher than that with two-time dilution method. Compared to one-time dilution method, protein expressions of 125 ku and 90 ku were less than those with two-time dilution method in the components of pig sperm acrosomal membrane proteins, but the protein expressions of 120 ku, 48 ku and 36 ku were higher than those with two-time dilution. In conclusion, there were certain relationships between the boar sperm damage and the expression of acrosomal membrane proteins of 125 ku, 120 ku, 90 ku, 48 ku and 36 ku in the frozen-thawed process of boar sperm.