The study was conducted to examine the effects of incubation time before the electrofusion and activation time of 6-DMAP on the development of the goat nuclear transplanted embryos in vitro. The reconstructed oocytes were continually cultured in a maturation medium for 30 min, 90 min and 9-10 h before the electrofusion, respectively. The results showed that the mortality of reconstructed oocytes of 9-10 h group after the electrofusion was significantly lower than those of 30 min and 90 min groups (27.42% vs. 49.26% and 56.3%, P<0.05), while the cleavage rate of 9-10 h group was significantly higher than those of 30 min and 90 min groups (68.15% vs. 55.6% and 54.24%, P<0.05). The nuclear transplanted embryos activated by ionomycin were activated with 6-DMAP for 0 h, 2 h, 3 h, 4 h, 5 h, and 8-10 h respectively. The results showed that the cleavage rate was not significantly different among groups (P>0.05), but it was significantly higher than that of the embryos without 6-DMAP (P<0.05). In conclusion, the continued incubation of reconstructed oocytes before electrofusion may be helpful for lowing the mortality of fusion and increasing the rate of available oocyte; the combination of ionomycin with 6-DMAP could improve the activation efficiency in goat nuclear transplanted embryos, and activation time of 6-DMAP has no significant effects on the cleavage of goat nuclear transplanted embryos.