Extensive use of Rht-B1b, Rht-D1b and Rht8 dwarf genes can not only enhance wheat lodging resistance, but also increase wheat yield. Clear distribution dwarf genes will provide molecular information in wheat dwarf breeding. In this article, we detected the distribution of Rht-B1a, Rht-B1b, Rht-D1b and Rht8 gene in 257 wheat varieties using STS and SSR molecular markers. The results showed that the frequency of Rht8 gene (106 wheat varieties, 41.2%) was the highest, Rht-D1b was higher (88 wheat varieties, 34.2%) and Rht-B1b was the lowest (70 wheat varieties, 27.2%) in 257 wheat resources. The research also showed that part of wheat resources contained different kinds of dwarfing gene combination, and their distribution frequencies were also different. Twenty five materials (9.7%) were detected to contain Rht-D1b and Rht8. Twenty four materials (9.3%) were detected to have Rht-B1b and Rht8. Nine materials (3.5%) were detected to carry Rht-D1b and Rht-B1b. Five materials (1.9%) were detected to contain Rht-B1b, Rht-D1b and Rht8. The results may provide important information for wheat lodging resistance breeding and dwarfing breeding.