The plasmid pTR102 with luxAB gene was successfully introduced in Brevundimonas diminuta L19 strain by electroporation to monitor colonization dynamics of the strain in the natural environment. The influencing factors, such as pulse field strength, pulse time and optical density at λ_{600 nm} of the culture on transformation efficiency were tested. The luxAB gene was expressed in the transformant strain L19-luxAB, and after continual transfer for 15 generations, the characteristic of luminescence was still stable. The survival conditions for L19-luxAB in soil were studied. The results showed that L19-luxAB stably colonized in the soil after 40 d and the L19-luxAB strain had high ability in degradation of malathion in soil.