Atractylodis macrocephalae, Angelica?dahurica and Platycodon grandiforus are important?Chinese herbal medicine plants and their rhizomes?have high?medicinal value. To clarify these Meloidogyne species infesting the three herbal medicine plants in Bozhou City, Anhui Province, the species of the root-knot nematodes on these plants were identified based on the morphological character of perineal patterns, biochemical character of esterase isozyme and molecular character of rDNA-ITS, 28S rD2/D3 and rDNA-IGS methods. The nematode species on A. macrocephalae and A.dahurica was identified as M. arenaria and the nematode species on P. grandiforus as M. incognita. This is the first report of M. arenaria on A. macrocephalae and A.dahurica in China. This result will be helpful to control the root-knot nematode disease of the three herbal medicine plants.