The incidences of anthracnose (Colletotrichum gloeosporioides) were investigated by periodically fixed plants observation to screen out plants with different infected degrees as experimental material. In different months of the growing season, fruit inclusions (tannin, anthocyanins), pH, buffer capacity of different disease resistance were determined and analyzed, and the results were as follows. In the seasonal occurrence of anthracnose, the relations between the incidence rate and plant physiological and biochemical characteristics indicated that the fruit tannins content in every tea oil variety was negatively correlated with the fruit maturity and the incidence, with that in disease-resistant varieties been 0.33% higher than that in the susceptible varieties. The content of anthocyanin in fruit coat of disease-resistant variety (11-19.5) was higher than that in susceptible variety (6.8-10.3), which was positively correlated with the maturity of the fruit. pH value in disease-resistant variety was less than 5, lower than that in susceptible one. Moreover, buffer capacity in disease-resistant variety (5.33-7.48) was lower than that in susceptible one (5.45-7.12).