皖南黑猪CETP基因单核苷酸多态检测与遗传分析
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国家自然科学基金(31171200), 科技富民强县专项行动计划, 国家农业科技成果转化资金(2011GB2C300017), 省级农业科技专家大院(2012)和科技部星火计划重点项目(2010GA710001)共同资助。


Single nucleotide polymorphism detection and genetic analysis of CETP gene in Wannan Black Pig
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    摘要:

    以 112头皖南黑猪为研究对象,采用PCR-SSCP方法检测胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)基因的多态性。结果表明:(1) 皖南黑猪CETP基因第1内含子795碱基处发生C→T突变(C795T);(2) 在C795T突变位点中检测到CC、CT、TT 3种基因型,其基因型频率分别为0.6250、0.3036和0.0714;C的基因频率为0.7768,T的基因频率为0.2232;(3) 皖南黑猪CETP基因的多态信息含量(PIC)为0.2866,该位点处于中度多态;卡方检验表明,该位点的χ2值为1.7388,处于哈迪-温伯格平衡状态。

    Abstract:

    One hundred and twelve pigs from Wannan Black population were used for detecting the polymorphism of CETP gene by PCR-SSCP method. The results show as follows. (1) C>T mutation (C795T) occurred in 795 base position of intron 1; (2) Three genotypes (CC, CT and TT) were found in C795T site, genotypic frequency of CC, CT and TT was 0.6250, 0.3036 and 0.0714, respectively, gene frequency of C and T was 0.7768 and 0.2232, respectively; (3) The polymorphism information content (PIC) was 0.2866 in Wannan Black population, which indicated it was moderate polymorphism. The χ2 value was 1.7388, which was in Hardy-Weinberg equilibrium in Wannan Black population (P>0. 05).

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  • 在线发布日期: 2016-12-06