国家自然科学基金（31171200）, 科技富民强县专项行动计划, 国家农业科技成果转化资金（2011GB2C300017）, 省级农业科技专家大院（2012）和科技部星火计划重点项目（2010GA710001）共同资助。
以皖南黑猪、安庆六白猪、圩猪和霍寿黑猪共236头猪为研究对象，采用PCR-SSCP方法检测猪脂滴包被蛋白（Perilipin, PLIN1）基因的遗传多态性。结果表明，在皖南黑猪、安庆六白猪PLIN1基因3 519 bp碱基处发生A>G突变，检测到AA、AB、BB 3种基因型, 其中AA基因型频率在这2个群体中分别为8.97%和29.23%，AB基因型频率分别为43.59%和56.92%，BB基因型频率分别为47.44% 和13.85%；皖南黑猪、安庆六白猪的多态信息含量（PIC）分别为0.3353和0.3690，在该位点均处于中度多态；卡方检验表明皖南黑猪群体和安庆六白猪群体均处于哈迪-温伯格平衡状态(P>0.05)；在圩猪、霍寿黑猪群体中PLIN1基因的第2外显子没有检测到突变。PLIN1基因的第3外显子在4个群体中均没有检测到突变。
Two hundred and thirty-six pigs from Wannan Black pig, Anqing Liubai pig, Wei Pig and Huoshou Black pig populations were used for detecting the polymorphism of PLIN1 gene by PCR-SSCP method. The results showed that a A>G mutation at 3 519 base position, and 3 genotypes (AA, AB and BB) were found in Wannan Black and Anqing Liubai. The genotypic frequency of AA was 8.97%, 29.23%, respectively, AB was 43.59%, 56.92%, respectively, and BB was 47.44%, 13.85%, respectively. The polymorphism information content (PIC) was 0.3353 in Wannan Black population and 0.3690 in Anqing Liubai population, which were all in average polymorphism situation. According to the χ2 test, there were in Hardy-Weinberg equilibrium in Wannan Black and Anqing Liubai population (P>0.05), but there was no mutation in exon 2 in Wei Pig and Huoshou Black pig populations. Moreover, there was no mutation in exon 3 in these four populations.