Two hundred and thirty-six pigs from Wannan Black pig, Anqing Liubai pig, Wei Pig and Huoshou Black pig populations were used for detecting the polymorphism of PLIN1 gene by PCR-SSCP method. The results showed that a A>G mutation at 3 519 base position, and 3 genotypes (AA, AB and BB) were found in Wannan Black and Anqing Liubai. The genotypic frequency of AA was 8.97%, 29.23%, respectively, AB was 43.59%, 56.92%, respectively, and BB was 47.44%, 13.85%, respectively. The polymorphism information content (PIC) was 0.3353 in Wannan Black population and 0.3690 in Anqing Liubai population, which were all in average polymorphism situation. According to the χ² test, there were in Hardy-Weinberg equilibrium in Wannan Black and Anqing Liubai population (P>0.05), but there was no mutation in exon 2 in Wei Pig and Huoshou Black pig populations. Moreover, there was no mutation in exon 3 in these four populations.