It is the precondition for improving conidia production to screen the best conidia medium for biocontrol fungi during the mass production of fungal conidia. In this study, cheap and convenient experimental materials were used to make solid culture medium, and on the basis of single factor experiment, quadratic general rotary unitized design was used to optimize the composition and proportion of solid medium which influenced solid fermentation stage conidia production amount of Metarhizium flavoviride Mf82, and the quality indicators of conidia powder were further tested. Results showed that in single factor experiment, conidia production of Metarhizium flavoviride Mf82 was of the highest amount, when flour, beans shell and tenebrio slough with proportion of 4:1:0.2 were selected from rice meal, flour, corn starch, beans shell and tenebrio slough; further quadratic general rotary unitized design showed that conidia production of Metarhizium flavoviride was of the highest amount of 2.39×10¹² conidia per kg medium, when ratio of flour, beans shell and tenebrio slough in the solid medium was 4:1:0.25; quality testing indicators showed that Metarhizium flavoviride conidia powder produced by this medium formula had reached the enterprise standard for fungi conidia powder production.