Dry seeds of wheat HEKE2 were radiated by ⁶⁰Co γ-ray with the dose of 300 Gy, and the genetic variation of protein content, gluten content, zeleny and hardness of 234 lines were analyzed in M₄ population. The contents of protein and gluten in 6 positive and 3 negative mutant lines were identified based on the selection criterion of over mean±2s. Further, their quality traits and farinogram parameters were detected, and high molecular weight glutenins（HMW-GS）were identified by SDS - PAGE in M₅ generation. The result showed that the farinograph map and farinogram parameters of the mutant line 242 changed significantly, and HMW-GS composition of the parent was 2+7+8+9+12, while it was 2+7+8+9 for the mutant line 108 and 240. The obvious change of HMW-GS composition suggests that mutations of glutenin locus may be occurred in the mutant lines.