以京411/红芒春21构建的重组自交系群体（RILs）为实验材料（包括177个家系），研究花后旗叶叶绿素含量及其与粒重的关系，并进行QTL定位。结果表明，2011和2012年，花后7 d（I期）、14 d（II期）、19 d（III期）旗叶叶绿素含量及总叶绿素含量与千粒重呈显著或极显著正相关。连锁分析及QTL定位结果表明，2AS2存在一新的QTL位点（Barc5~Gwm448），控制I期和II期的旗叶叶绿素含量，于2011年和2012年均被检测到，可分别解释I、II时期叶绿素含量表型变异的16.38%~20.12%和17.27%~19.35%。而另一位点2AS1（Barc1138~Barc212）只在2011年检测到，可分别解释I、II期叶绿素含量变异的11.43%和9.67%。同时，该新位点（Barc5~Gwm448）也与千粒重变异有关，可分别解释其表型变异的13.35%（2011年）和10.14%（2012年）。未检测到花后其他时期旗叶叶绿素含量的基因位点。
The correlation between chlorophyll content of flag leaf after anthesis from 7 days to 24 days and 1000-kernal weight (TKW) was analysed using a RILs population from the cross between Jing411 and Hongmangchun21. In addition, the QTLs controlling these traits were also located according to the data of 2011 and 2012 crop seasons. The results indicated that the chlorophyll contents of flag leaf of 7 days (I), 14 days (II), 19 days (III) after anthesis and the total contents were positively related with TKW at the levels of 0.05 and 0.01 respectively in the two crop season. And the analysis of molecular markers linkage indicated a new QTL was found on the 2AS linked to the markers of Barc5 and Gwm448, which could explain 16.38%-20.12% (I) and 17.27%-19.35% (II) phenotype variations of chlorophyll content of flag leaf at 2011 and 2012 crop seasons respectively. Another QTL located between Barc1138 and Barc212 was only detected at 2011 crop season, which could explain 11.43% (I) and 9.67% (II) phenotype variations of chlorophyll content of flag leaf respectively. Simultaneously, the new QTL between Barc5 and Gwm448 was also confirmed to correlate with TKW and explained the variations of 13.35% (2011) and 10.14% (2012) respectively. No loci were found for the chlorophyll content of the flag leaf at other stags after anthesis.