One hundred and twenty-eight cattles from Wandong and Guangfeng populations were used to detect the polymorphism of MyoG gene by PCR-RFLP method. The results show that a G>C mutation was occur in 466 base position of exon 1, and 2 types of genotypes (AA and AB) were found. The mutation had not caused the change of amino acid. Genotypic frequencies of AA and AB are 23.66% and 76.34% respectively in Wandong population, the genotypic frequency of AA is 17.24% and AB is 82.76% in Guangfeng population. The polymorphism information content (PIC) is 0.3606 in Wandong population and 0.3702 in Guangfeng population, which are all in average polymorphism situation. According to the χ² test, there is not in Hardy-Weinberg equilibrium in Wandong population (P<0.05) and there is in Hardy-Weinberg equilibrium in Guangfeng population (P>0. 05).