In order to investigate the relationship between antimicrobial resistance and serotypes, genotypes of Salmonella isolates from apparently healthy chickens in Hefei, the Kirby-Bauer method was applied to determine the sensitivity of 21 strains susceptibility to 21 antibiotics, and PCR technique also was used to detect 32 kinds of drug-resistance genes, based on identified serotypes and ERIC-PCR gene-type. It was found that 100%(21/21)of the Salmonella isolates were resistant to all antibiotics by Drug Susceptibility Test; 76.19% (16/21) were resistant to at least two antimicrobials among the 21 isolates, and the dominating multidrug-resistant spectrum was Penicillin G-Ampicillin-Amoxicillin-Streptomycin-Trimethoprim-sulfamethoxazole. It was the most extensive in resistance to β-lactamase antibiotics by 100%(21/21). Meanwhile, the resistance genes could be detected from all these isolates by PCR, including bla_PSE, sul1, strA, bla_TEM, sul2, tetA, tetB, aph3-Ⅱa and catⅠ, and bla_PSE gene was the most prevalent with the detected rate of 85.7%(8/21). It is concluded that the stronggest antimicrobial resistance to β-lactamases drugs on Salmonella isolated from apparently healthy chickens in Hefei due to the existence of bla_PSE gene; antimicrobial phenotype is also related to the antimicrobial resistance genes; antimicrobial resistance is in relevant with the same sources of the same serotypes and genotypes.