研究了培养液组分浓度、培养方法和卵母细胞来源对黄牛卵母细胞第一极体（PB1）排出的影响。从屠宰场收集黄牛卵巢，使用抽吸法获取卵丘卵母细胞复合物（COCs）进行体外培养。结果表明，采用以下培养条件能够显著促进黄牛卵母细胞PB1排出：在培养液中添加10 μg·mL-1 FSH和LH、1.0 μg·mL-1 17-β E2、33.0 μg·mL-1丙酮酸钠；培养时间24 h，培养密度每50 μL培养液培养10~20枚COCs；卵巢储运温度25～37 ℃，储运时间<3 h，卵泡直径3～6 mm，卵丘细胞包裹2层以上。选择最佳的培养液组分浓度、培养方法和卵母细胞来源可获得最大PB1排出量，为下一步利用PB1进行保护物种、扩大优良母畜遗传资源的来源和植入前遗传学诊断等研究提供参考。
For investigating PB1 extrusion of cattle’s oocytes under different component concentrations of culture solution, culture methods in vitro and oocytes derivation, COCs suctioned from abattoir-derived ovarian follicles were cultured under different situations. Results showed that the culture conditions in vitro promoted PB1 extrusion of cattle’s oocytes significantly (P<0.05). The optimized components added in the culture medium were 10 μg·mL-1 FSH, 10 μg·mL-1 LH, 1.0 μg·mL-1 17-β E2 and 33.0 μg·mL-1 sodium pyruvate, and the optimized culture time in vitro was 24 h; culture density was 10-20 COCs per 50 μL of the culture solution; temperature of ovaries storage and transportation were at 25-37℃, and transportation time was less than 3 h; the ovarian follicle diameter was 3-6 mm; and the oocytes covered 2 layer cumulus cells would well matured. The maximum available PB1 provided research basis for protecting species, improving genetic resources of excellent females and preimplantation genetic diagnosis in the future.