For investigating PB1 extrusion of cattle’s oocytes under different component concentrations of culture solution, culture methods in vitro and oocytes derivation, COCs suctioned from abattoir-derived ovarian follicles were cultured under different situations. Results showed that the culture conditions in vitro promoted PB1 extrusion of cattle’s oocytes significantly (P<0.05). The optimized components added in the culture medium were 10 μg·mL^-1 FSH, 10 μg·mL^-1 LH, 1.0 μg·mL^-1 17-β E₂ and 33.0 μg·mL^-1 sodium pyruvate, and the optimized culture time in vitro was 24 h; culture density was 10-20 COCs per 50 μL of the culture solution; temperature of ovaries storage and transportation were at 25-37℃, and transportation time was less than 3 h; the ovarian follicle diameter was 3-6 mm; and the oocytes covered 2 layer cumulus cells would well matured. The maximum available PB1 provided research basis for protecting species, improving genetic resources of excellent females and preimplantation genetic diagnosis in the future.