Abstract:The plant expression vector pCUB-Zein::ferritin-35s::bar was constructed by In-Fusion kit. The soybean ferritin gene was transferred into the shoot apical point of maize inbred line of maize inbred line Qi319 by Agrobacterium tumefacien mediated transformation. The soybean ferritin gene was expressed under the control of a maize seed storage protein prolamin promoter 15kD β-Zein. 272 transgenic plants were obtained after selecting with glufosinate and 108 of them were confirmed positive by PCR amplification. The frequency of transformation reached about 3.6%. This study showed that the soybean ferritin gene was integrated into maize genome.