为了解小麦孢囊线虫 (Cereal cyst nematode, CCN) 在安徽省的分布和主要麦区CCN的种类，以及群体间关系，调查了淮北等麦区小麦根系白雌虫，用 “Zig-Zag” 法在怀远等地采集土样，用Fenwick & Oostenbrink法分离土样中的孢囊并孵化得2龄幼虫，利用孢囊阴门锥形态及de-man法鉴定CCN种类，扩增ITS特征片段，用MEGA5.05构建系统发育树。结果表明，安徽省52% 采样点检出 CCN，淮河以北采样点均检出CCN，江淮麦区7%的采样点检出 CCN，长江以南麦区采样点未检出 CCN。发生在怀远、凤台、灵璧、界首和颍上麦区小麦根际CCN鉴定为禾谷孢囊线虫（Heterodera avenae），淮河以北发生H. avenae麦区占淮北小麦种植面积的 41.6%，另外，怀远H. avenae群体和其他群体亲缘关系较远，群体间形态有一定差异，但和亲缘关系差异无一致性。
The purpose of this paper is to survey distribution, species of cereal cyst nematode (CCN) and relationship among CCN species in Anhui Province. White female of wheat root system in Huaibei wheat areas were investigated; soil samples of wheat rhizosphere were collected with "Zig-Zag" method in Huaiyuan county and other counties; cysts were separated by Fenwick & Oostenbrink method from those samples; second-stage juveniles (J2) were obtained by hatching cyst; CCN was identified based on morphologic characteristics of cyst perinea pattern and de-man data; and the genetic relationships were analyzed by amplified ITS characteristic fragment and phylogenetic tree built with MEGA5.05. The results showed that CCN was detected from 52% sample sites, from all the samples of wheat areas in the northern part of the Huai River, and from 7% sample sites in Jianghuai wheat areas. No CCN was detected from wheat areas in the southern part of the Changjiang River. Occurrence of CCN in Yingshang and other 4 counties were identified as Heterodera avenae, and occurrence area of H. avenae was up to 41.6% of wheat areas in the northern part of the Huai River. Huaiyuan population of H.avenae is distantly related with other 4 H.avenae populations, and certain morphological differences exist among the 5 H.avenae populations, but with no consistency to the genetic relationships.