运用荧光标记微卫星分析6个肉山羊品种遗传多样性
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转基因生物新品种培育重大专项(2009ZX08008-007B), 国家高技术研究发展计划(863)(2011AA100307), 安徽省技术创新工程试点省专项(11Z0101074)和安徽省科技计划项目(11010302108)共同资助。


Genetic diversity of six meat goat breeds revealed by fluorescent microsatellite markers
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    摘要:

    为了研究安徽白山羊、贵州白山羊、陕南白山羊、宜昌白山羊、马头山羊和波尔山羊6个肉山羊品种DNA分子水平上的遗传多样性,利用10个微卫星引物,分析6个肉山羊品种分子水平上的遗传变异和群体关系。10个微卫星位点均呈现高度多态,其多态信息含量(PIC)在0.531~0.812之间。F统计结果显示群体间存在极显著的遗传分化,遗传分化系数(Fst)为0.082。系统进化树NJ图把中国5个肉山羊和波尔山羊分为2支;中国肉山羊又被分成2个分支。系统进化树UPGMA图和主成分的二维坐标图进一步证实上述聚类结果的可靠性。此研究结果显示6个肉山羊品种遗传多样性丰富,具有很大的选育潜力,特别是中国地方品种,是将来开发利用的宝贵原始材料。

    Abstract:

    Genetic variation of 5 Chinese indigenous meat goat breeds and one introduced breed were examined using 10 microsatellite DNA markers.Ten of all the microsatellite loci were highly polymorphic and effective markers for analysis of genetic diversity and relationship among goat populations. The result showed that 6 meat goat breeds have high genetic diversity. The genetic differentiation was low between meat goat breeds which displayed only 8.2% of the total genetic variance among the different breeds. Neighbour-joining (NJ) dendrogram clustered Chinese indigenous meat goat breeds and Boer goat into different groups. Furthermore, Chinese indigenous meat goat breeds were grouped into several different phylogenetic clusters which corresponded to the geographic regions. This grouping pattern was further supported by both UPGMA dendrogram and scatter plots of estimates from principal component analyses. These results may provide a scientific basis for the characteristization, conservation and utilization of Chinese meat goats.

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  • 在线发布日期: 2016-12-05