The shoots and young leaves of Aechmea fasciatacv were used as cell culture material with MS medium plus different concentration hormones. The results show that MS+BA1.0 mg·L^-1 + IBA 0.1 mg·L^-1 is the best medium for shoots induction; MS+BA 0.2 mg·L^-1 +NAA 0.1 mg·L^-1 is the best medium for shoot propagation, whereas 1/2MS+NAA 0.5 mg·L^-1 is suitable medium for roots induction. PCR amplification shows that genomic DNA of regenerated plants is not obviously different from original Aechmea fasciatacv.