In the present study, the abilities of episodic-like memory in 13- and 5-month-old CD-1 mice were explored using a three-trial object exploration task. The results showed that the 13-month-old mice had significantly lower preference index for exploring the spatially displaced “old familiar” object relative to the 5-month-old mice (P < 0.05). However, there were no significant inter-group differences in the preference index exploring both “old familiar” objects and the total time exploring all of the four objects (Ps > 0.05). Furthermore, neither of the sex and the interaction of age × sex significantly affected these preference indexes and the total time (Ps > 0.05). Our results indicated that the middle-aged CD-1 mice had declined episodic-like memory, which might be attributable to the component of “where” in the episodic-like memory task.