在基础饲料中分别添加番茄红素（lycopene，LCP）含量为0.50%、0.75%和1.00%的番茄红素油1.5%，对照组为1.5%的鱼油，即饲料中番茄红素添加量为0、75.0、112.5、150.0 mg·kg-1。人工投喂饲养平均体长22.4±1.13 cm，平均体重180.60±37.23 g的鲫鱼50 d。每网箱随机取样20尾（n=20）试验鱼，进行血清总超氧化歧化酶活性（T-SOD）、丙二醛含量（MDA）、谷胱甘肽过氧化物酶活力（GSH-Px）、一氧化氮含量（NO）和抑制羟自由基能力（·OH）测定，以研究番茄红素对养殖鲫鱼抗氧化能力的影响。试验数据以平均值±标准差（Mean±SD）表示，SPSS11.5软件Duncan多重比较检验。结果显示，添加番茄红素试验组血清T-SOD活性比对照组分别提高了8.49、43.33和48.57 U·mL-1，番茄红素添加量112.5和150.0 mg·kg-1组的血清T-SOD活性与对照组比较有显著差异（P < 0.05）。添加番茄红素试验组血清中MDA含量分别比对照组降低了3.75、4.55和2.70 nmol·mL-1，添加番茄红素和试验组与对照组比较血清MDA含量均无显著差异（P > 0.05）。添加番茄红素试验组血清GSH-Px活力分别比对照组提高了99.32、138.77和122.61 U，添加番茄红素的各试验组与对照组比较血清GSH-Px活力均有显著差异（P < 0.05）。添加番茄红素试验组血清NO含量分别高于对照组12.83、8.30和13.02 μmol·mL-1，添加番茄红素75.0和150.0 mg·kg-1组与对照组比较血清NO含量有显著差异（P < 0.05）。添加番茄红素试验组血清中抑制·OH能力比对照组降低了318.50、290.91和277.99 U·mL-1，添加番茄红素75.0 mg·kg-1组与对照组比较血清中抑制·OH能力差异显著（P < 0.05）。添加番茄红素各试验组间比较T-SOD活性、GSH-Px活力、MDA含量、NO含量和抑制·OH能力均无显著差异（P < 0.05）。综合试验结果，在鲫鱼人工配合饲料的油脂中添加番茄红素，有效地提高了养殖鲫鱼内源性抗氧化物质的水平，实现了抗氧化剂的抗氧化作用。
Effects of lycopene supplements on antioxidant capacity were studied in Crucian Carp, Carassius auratus, with average length 22.4±1.13 cm and weight 180.60 ± 37.23 g. Fish were fed a commercial diet supplemented with Lycopene at levels of 0.50%, 0.75%, 1.00%, and with 1.5% fish oil as the control in cages for 50 days. That is, four diets contained Lycopene at 0, 75, 112.5, and 150 mg·kg-1 for a control diet and other three diets containing Lycopene. Twenty fish was randomly taken from each cage. As biomarkers, T-SOD, MDA, GSH-Px, NO, and ·OH were measured in blood serum. The experimental data were presented in mean ± standard deviation (SD). Duncan test was performed for the multiple comparisons among four diets using SPSS 11.5 version. The study shows: The levels of T-SOD in fish blood serum were 8.49, 43.33, and 48.57 U·mL-1 higher in fish fed with three diets added lycopene than that of the control. The levels of T-SOD were significantly higher (P<0.05) in fish fed with diet added 112.5 and 150.0 mg·kg-1 lycopene than that of the control. The levels of MDA in the blood serum of fish fed with diets were 3.75, 4.55, and 2.70 nmol·mL-1 lower than that of control. But, no significant difference was found in levels of MDA in fish fed diets added 75.0, 112.5, 150.0 mg·kg-1 lycopene oil and the control. The levels of GSH-Px activity in the blood serum of fish fed with three lycopene diets were 99.32, 138.77,122.61 U higher compared to the control. The GSH-Px activity in blood serum of fish fed diets added lycopene was significantly higher (P<0.05) than that of the control. The levels of NO in fish fed diets adding lycopene were 12.83, 8.30, and 13.02 μmol·mL-1 higher than that of control. The levels of NO in fish fed 75 and 150 mg·kg-1 lycopene were significantly different (P<0.05) than that of control fish. The activities of ·OH in fish fed lycopene were 318.50, 290.91, and 277.99 U·mL-1 lower than that of control. The activity of ·OH was significantly decreased (P<0.05) in fish fed diet added 75.0 mg·kg-1 lycopene than that of the control. No significant differences were found in levels of T-SOD, GSH-Px, MDA, NO, and ·OH in blood serum of fish fed diets added with 75.0, 112.5, 150.0 mg·kg-1 lycopene. The experiment demonstrated that lycopene as an antioxidant added to a commercial diet can significantly improve the antioxidant capacity of Crucian Carp.